Cold Storage of Isolated Class C Chloroplasts
نویسندگان
چکیده
منابع مشابه
Isolated Chloroplasts
1. Chloroplasts prepared by the non-aqueous technique will, after fragmentation by ultrasonic treatment, incorporate [2-14C]mevalonic acid into phytoene, the first C40 compound formed in the biosynthetic sequence to coloured carotenoids. 2. With suspensions containing 3*5mg. of chlorophyll, the optimum amounts of cofactor required were ATP (10,umoles), magnesium chloride (20,umoles) and glutath...
متن کاملPhotosynthesis by isolated chloroplasts.
Photosynthesis in green plants requires not only photoelectron transport, oxygen evolution, and photophosphorylation, but also the synthetic reactions whereby carbon dioxide is assimilated and reduced to a number of organic compounds via the photosynthetic carbon reduction cycle1 and secondary biosynthetic pathways.2 Complete photosynthesis with isolated chloroplasts has for many years been a g...
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The ability of chloroplasts isolated from Acetabulana mediterranea to synthesize the protein amino acids has been investigated. When this chloroplast isolate was presented with (14)CO(2) for periods of 6-8 hr, tracer was found in essentially all amino acid species of their hydrolyzed protein Phenylalanine labeling was not detected, probably due to technical problems, and hydroxyproline labeling...
متن کاملSynthesis of Chloroplast Dna in Isolated Chloroplasts
Chloroplasts isolated from Euglena gracilis incorporated both tritiated thymidine 5'-triphosphate and tritiated deoxyadenosine 5'-triphosphate into an acid-stable fraction. The incorporation was dependent on the presence of all four deoxynucleoside triphosphates and was sensitive to treatment with deoxyribonuclease and actinomycin D. It was demonstrated that bacterial contamination could not ac...
متن کاملUltrastructure of Chloroplasts Isolated by Nonaqueous Extraction
INTRODUCTION been carried out with anthocyanin as a marker of vacuolar material and with various enzymes as markers of mitochondrial and hyaloplasmic commethod of isolating cell organelles (2) and its markers of mitochondrial and hyaloplasmic commodification for use in isolating chloroplasts (3, ponents 5, 13), it has been used extensively in the study visual observations of the purity of these...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1979
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.64.6.942